Tuesday, March 04, 2008

Plant Flow Cytometry Groups - Portugal

A new section regarding the plant flow cytometry groups of each country is starting this month. A new country/region will be posted each month.

To start, and despite I am currently located in Czech Republic, the home country of the administrators of this blog was chosen: PORTUGAL

Unfortunately, so far, only a few groups in Portugal have used flow cytometry to study plant cells. Their list follows below:

Conceição Santos, João Loureiro (now in Department of Botany, Charles University in Prague, Czech Republic), Eleazar Rodriguez, Glória Pinto, Sílvia Castro - Laboratory of Biotechnology and Cytomics, Department of Biology, University of Aveiro, Aveiro, Portugal
No. of publications: 10 (1 in collaboration with Fernanda Leal and Olinda Pinto-Carnide, Department of Biotechnology and Genetics, University of Trás Montes & Alto Douro, Vila Real, Portugal)

Maria Lima and Maria Candeias - National Agronomic Station, Department of Plant Protection, Oeiras, Portugal (flow cytometry with collaboration of Artur Paiva, Centro Histocompatibilidade do Centro, Coimbra, Portugal)
No. of publications: 1

Augusto Peixe - University of Évora, Évora, Portugal (flow cytometry services provided by the Plant Cytometry Services – Schijndel, The Netherlands)
No. of publications: 2

Ana Delauney Caperta - Centro de Botânica Aplicada à Agricultura, Instituto Superior de Agronomia, Universidade Técnica de Lisboa, Lisbon (flow cytometry with collaboration of Armin Meister, IPK, Department of Cytogenetics, Gatersleben, Germany)
No. of publications: 1

Flow cytometry core facilities in Portugal:
Cell Imaging Unit - Instituto Gulbenkian de Ciência, Lisbon (Responsible for all flow cytometry services: Rui Gardner)
Flow Cytometry Unit - Institute for Molecular and Cell Biology, Porto (Responsible: Simon Monard)
Flow Cytometry Unit - Institute of Molecular Medicine, Lisbon

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Reference list:
Caperta A. D., Delgado M., Ressurreicao F., Meister A., Jones R. N., Viegas W. & Houben A. (2006): Colchicine-induced polyploidization depends on tubulin polymerization in c-metaphase cells. – Protoplasma 227: 147-153.

Castro S., Loureiro J., Rodriguez E., Silveira P., Navarro L. & Santos C. (2007): Evaluation of polysomaty and estimation of genome size in Polygala vayredae and P-calcarea using flow cytometry. – Plant Science 172: 1131-1137.

Castro S., Loureiro J., Santos C., Ater M., Ayensa G. & Navarro L. (2007): Distribution of flower morphs, ploidy level and sexual reproduction of the invasive weed Oxalis pes-caprae in the western area of the Mediterranean region. – Annals of Botany 99: 507-517.

Conde P., Loureiro J. & Santos C. (2004): Somatic embryogenesis and plant regeneration from leaves of Ulmus minor Mill. – Plant Cell Reports 22: 632-639.

Greilhuber J., Temsch E. & Loureiro J. (2007): Nuclear DNA Content Measurement. In: – Dolezel J., Greilhuber J. & Suda J. (eds) Flow cytometry with plant cells. Wiley-Vch Verlag, Weinheim, pp 67-101.

Leal F., Loureiro J., Rodriguez E., Pais M. S., Santos C. & Pinto-Carnide O. (2006): Nuclear DNA content of Vitis vinifera cultivars and ploidy level analyses of somatic embryo-derived plants obtained from anther culture. – Plant Cell Reports 25: 978-985.

Lima M., Paiva A. & Candeias M. I. (2003): Flow cytometry - a simple method for nuclear DNA content evaluation of Vitis vinifera cv. Periquita somatic embryos obtained from anther cultures. – Vitis 42: 99-100.

Loureiro J., Pinto G., Lopes T., Dolezel J. & Santos C. (2005): Assessment of ploidy stability of the somatic embryogenesis process in Quercus suber L. using flow cytometry. – Planta 221: 815-822.

Loureiro J., Rodriguez E., Costa A. & Santos C. (2007): Nuclear DNA content estimations in wild olive (Olea europaea L. ssp europaea var. sylvestris Brot.) and Portuguese cultivars of O. europaea using flow cytometry. – Genetic Resources and Crop Evolution 54: 21-25.

Loureiro J., Rodriguez E., Dolezel J. & Santos C. (2006): Comparison of four nuclear isolation buffers for plant DNA flow cytometry. – Annals of Botany 98: 679-689.

Loureiro J., Rodriguez E., Dolezel J. & Santos C. (2006): Flow cytometric and microscopic analysis of the effect of tannic acid on plant nuclei and estimation of DNA content. – Annals of Botany 98: 515-527.

Loureiro J., Suda J., Dolezel J. & Santos C. (2007): FLOWer: A plant DNA flow cytometry database. In: – Dolezel J., Greilhuber J. & Suda J. (eds) Flow cytometry with plant cells. Wiley-VCH, Weinheim, pp 423-438.

Peixe A., Barroso J., Potes A. & Pais M. S. (2004): Induction of haploid morphogenic calluses from in vitro cultured anthers of Prunus Armeniaca cv. 'Harcot'. – Plant Cell Tissue and Organ Culture 77: 35-41.

Peixe A., Campos M. D., Cavaleiro C., Barroso J. & Pais M. S. (2000): Gamma-irradiated pollen induces the formation of 2n endosperm and abnormal embryo development in European plum (Prunus domestica L., cv, "Rainha Claudia Verde"). – Scientia Horticulturae 86: 267-278.

Pinto G., Loureiro J., Lopes T. & Santos C. (2004): Analysis of the genetic stability of Eucalyptus globulus Labill. somatic embryos by flow cytometry. – Theoretical and Applied Genetics 109: 580-587.

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