The return of the blog

Dear friends,

I know! It has been already more than two months without any posts in the blog. Since the Symposium of the International Organization of Plant Biosystematics in Slovakia that I've been most of the time out of office in several field trips for collection of Aster amellus. This beautiful daisy has been studied throughly by Zuzana Münzbergova in the Czech Republic where a secondary contact zone between two cytotypes (diploids and hexaploids) can be found. In order to understand the distribution of both cytotypes Sílvia Castro and I have been collecting this species in the neighbouring countries of the Czech Republic. So far, we have thoroughly explored the Slovak Republic and Austria, but there's still Poland to come. However, before leaving to the Polish land for the last week of field season of this year I will post the usual Articles of the Month section and one of several software highlights.

Xth Symposium of the International Organization of Plant Biosystematics

The next Symposium of the International Organization of Plant Biosystematics will occur next week (from the 2nd to the 4th of July) in Visoké Tatry, Slovakia. This year the meeting will be devoted to the evolution of plants in montainous and alpine habitats. The impact that flow cytometry has been having in this area is well patent in the Symposium Scientific Programme as there will be one talk totally dedicated to it (Jan Suda, Praha - From individuals to populations: the impact of flow cytometry on understanding polyploid evolution in mountain plants) and some more talks that will certainly focus on data obtained using this powerful and high throughput technique.

The main topics that will be focused are:
PHYLOGEOGRAPHY
BIOGEOGRAPHY
EVOLUTIONARY PROCESSES IN EUROPEAN MOUNTAIN RANGES
POLYPLOIDY
MOLECULAR APPROACHES IN PLANT EVOLUTION
ECOLOGICAL FACTORS IN PLANT EVOLUTION
EVOLUTIONARY PROCESSES IN EXTRA-EUROPEAN MOUNTAINS
ROLE OF APOMIXIS IN PLANT EVOLUTION

There is still the possibility to register on site, so do not miss this opportunity to, besides enjoying a Symposium with a rather interesting programme (which should be the main purpose!), discover this beautiful mountain area of Slovakia.

I hope to see you there.

Plant Flow Cytometry - Far Beyond the Stone Age Commentary on Cytometry Journal

In a close association with the review of Sergio Ochatt on the application of flow cytometry in plant breeding (see previous post) that appears in the current issue of Cytometry Part A journal we have been invited to write a commentary on the current state of flow cytometry in plant sciences. It is a short overview of the applications of flow cytometry to analyse plant cells and once again the blog, forum and FLOWer database were not forgotten. We hope that you enjoy the reading.

You can download it for free in here: Plant Flow Cytometry - Far Beyond the Stone Age

The first reference in a manuscript to the Plant FCM blog and forum

In the last issue of Cytometry a review on the application of flow cytometry to plant breeding is presented by Sergio J. Ochatt from INRA (Dijon Cedex, France). The manuscript explores some of the interesting uses of flow cytometry in the area of plant breeding and, by the end, in an overview of the progress in recent years, both this blog and the plant flow cytometry forum are referred with the respective web address. We are very thankful for this mention and it is already a victory that we are slowly gaining some visibility within the plant FCM community.

Thank you all for being so supportive.

ISAC Congress - Plant FCM Workshop Presentations

Despite that the Congress was already over almost one month ago, we are still summarizing some of the plant related events. Now, it is time to provide you with the presentations given in the plant FCM workshops (click on each image for download). The presentations may be used without copyright restrictions except for commercial or for profit use, provided there is some credit to the author and reference to this blog.

Workshop - Plant genome structure and gene expression - organized by David Galbraith and Jaroslav Dolezel











Presentations available by Jan Suda (Preparation and storage of plant samples for DNA flow cytometry), João Loureiro (The effect of cytosol on quantitative staining of nuclear DNA), Johann Greilhuber (Standardization and Standards), Jaroslav Dolezel (Chromosome analysis and sorting) and David Galbraith (Analysis of gene expression, including flow analysis and sorting of organelles and large cells)

Workshop - Plant evolutionary biology, biosystematics and ecology - organized by Brian Husband and Jan Suda







Presentations available by Jan Suda (The impact of FCM on plant Biosystematics and Taxonomy), Paul Kron (Flow Cytometry: Applications in the Study of Plant Reproductive Systems) and Brian Husband (Flow Cytometry and The Evolutionary Dynamics of Polyploids).

We hope that you enjoy the presentations.

Flow Cytometry Blog

While surfing the net for some information about FCM courses I found this blog (link provided bellow) that lists some of the courses that will be lectured this year.


There are not Plant FCM-specific courses listed, but the basis of FCM is the same for all the fields of biology, so if you are interested in learning more about FCM, you should try some of the courses listed in that page, some are hands-on so you will get a lot of practical knowledge as well.

http://www.squidoo.com/flowcytometrycourses

Cheers

List of recently published papers on plant flow cytometry - May

Below is the list of papers that came to our hands/knowledge during the month of April. In here there are already included some of the contributions made through the FLOWer database webpage. Many thanks for the authors that contributed with their work.

Genome size:
Carvalho CR, Clarindo WE, Praça MM, Araújo FS, Carels N. Genome size, base composition and karyotype of Jatropha curcas L., an important biofuel plant. Plant Science (2008) 174:613-617.

Rossi AAB, Clarindo WR, Carvalho CR, Oliveira LO. Karyotype and nuclear DNA content of Psychotria ipecacuanha: a medicinal species. Cytologia 73 (2008):53–60.

Ecology
Ayres DR, Grotkopp E, Zaremba K, Sloop CM, Blum MJ, Bailey JP, Anttila CK, Strong DR. Hybridization between invasive Spartina densiflora (Poaceae) and native S. foliosa in San Francisco Bay, California, USA. American Journal of Botany (2008) 95:713-719.

Biotechnology
Makowczynska J, Andrzejewska-Golec E, Sliwinska E. Nuclear DNA content in different plant materials of Plantago asiatica L. cultured in vitro. Plant Cell, Tissue and Organ Culture (2008) 94:65–71

Ploidy level:
Biotechnology
Kantartzi S, Roupakias DGProduction of aneuploids of the cotton hybrid G. barbadense x G. hirsutum L. via intergeneric pollination with Abelmoschus esculentus. Euphytica (2008) 161:319-327.

Orbovic V, Calovic M, Viloria Z, Nielsen B, Gmitter FG, Castle WS, Grosser JW. Analysis of genetic variability in various tissue culture-derived lemon plant populations using RAPD and flow cytometry. Euphytica (2008) 161:329-335.

Guillemin ML, Faugeron S, Destombe C, Viard F, Correa JA, Valero M. Genetic variation in wild and cultivated populations of the haploid-diploid red alga Gracilaria chilensis: how farming practices favor asexual reproduction and heterozygosity. Evolution (2008) 62-6: 1500–1519

Functional cytometry:
Apoptosis
Tilbrook J, Tyerman SD. Cell death in grape berries: varietal differences linked to xylem pressure and berry weight loss. Functional Plant Biology (2008) 35:173-184.